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Title: Cell Image Library Dataset CIL:10522      
dateReleased:
03-08-2011
description:
Time-lapse of Toxoplasma gondii. A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgIMC1 and mRFP-tagged TgMORN1 (MORN domain containing protein). Formation of daughter cells is visible during time-lapse. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. The nonfluorescent host fibroblasts are seen in the DIC image only. 3D stacks of images were collected at 0.6 μ increments in z, every 10 min. The DIC image is a single plane from the middle of the stack. Corresponding deconvolved stack: CIL# 10523 and projection of the deconvolved stack: CIL# 10524 and movie of projection: CIL# 10525 Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details.
privacy:
not applicable
aggregation:
instance of dataset
ID:
10522
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission, optical path length gradient
keywords:
EGFP; GFP; mRFP1
name:
live-cell imaging, living tissue
name:
cell division
name:
cytoskeletal organization and biogenesis
name:
cytoskeleton organization
name:
regulation of cell shape
name:
disseminated toxoplasmosis
name:
IDO:IDO:0000038
name:
toxoplasmosis
name:
Toxoplasma gondii RH stable cell line
name:
basal part of cell
name:
centriole
name:
conoid
name:
inner membrane complex
name:
spindle pole
name:
DIC, differential interference contrast microscopy, fluorescence microscopy, time lapse microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/10522.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/10522.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
public_domain
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/10522.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
public_domain
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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