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Title: Cell Image Library Dataset CIL:10362      
dateReleased:
12-10-2010
description:
Cultured hippocampal neurons after 17 days in vitro, immunostained for MAP2, a microtubule associated protein localized to dendrites (red) but not axons, which are not apparent in the immunofluorescence channel. Both axons, and dendrites, can be seen in the hidden phase micrograph which can be turned on using the edit function in the detailed viewer. Neurons at 10, 14, and 17 days in vitro are represented in this image group. Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for MAP2 (HM2, from Sigma with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 40X lens (HCX PL Fluotar, NA 0.75), Photometrics CoolSnap ES CCD camera and MetaMorph software. A multilayer stack of the fluorescent image of MAP2 staining and the phase image was generated using MetaMorph.
privacy:
not applicable
aggregation:
instance of dataset
ID:
10362
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
primary antibody plus labeled secondary antibody
name:
detergent permeabilized, formaldehyde fixed tissue
name:
multipolar neuron
name:
cellular developmental process
name:
dendrite development
name:
establishment and/or maintenance of cell polarity
name:
establishment or maintenance of cell polarity
name:
protein localisation to synapse
name:
protein localization to synapse
name:
synapse assembly
name:
synapse biogenesis
name:
dendrite
name:
microtubule cytoskeleton
name:
synapse part
name:
fluorescence microscopy, phase contrast microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/10362.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_cc_by
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/10362.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
attribution_cc_by
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/10362.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
attribution_cc_by
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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