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Title: Cell Image Library Dataset CIL:10276      
dateReleased:
12-23-2010
description:
After over-night serum starvation, mouse fibroblast expressing Dyn2-GFP were stimulated with 10ng/ml PDGF. After 5min, the cells were fixed and stained with rhodamine-phalloidin. PDGF-induced dorsal membrane ruffle was indicated by F-actin staining, and the enrichment of dynamin to the membrane ruffle was shown by their co-localization. Biological Source Mus musculus ES cell derived fibroblast PDGF-induced macropinocytosis PFA fixation Biological Context and Probe PDGF-induced dorsal membrane ruffle, Dyn2-GFP and rhodamine-phalloidin Equipment used Inverted Olympus IX-70 microscope with a 100×, 1.35 NA oil-immersion objective Magnification of image 1000x, scale bar indicates 5 um
privacy:
not applicable
aggregation:
instance of dataset
ID:
10276
refinement:
curated
availability:
available
types:
image
dimensions:
fluorescence emission
keywords:
EGFP; GFP; phalloidin; Rhodamine
name:
fibroblast
name:
clathrin-independent pinocytosis
name:
PDGF receptor signaling pathway
name:
pinocytosis
name:
platelet-derived growth factor receptor signaling pathway
name:
actin cytoskeleton
name:
dorsal membrane ruffle
name:
dynamin
name:
fluorescence microscopy
accessURL: www.cellimagelibrary.org/images/download_jpeg/10276.jpg
storedIn:
Cell Image Library
qualifier:
not compressed
license:
copyright
format:
JPGE
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/10276.tif
storedIn:
Cell Image Library
qualifier:
not compressed
license:
copyright
format:
OME-TIF
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
accessURL: http://grackle.crbs.ucsd.edu:8080/OmeroWebService/images/10276.raw
storedIn:
Cell Image Library
qualifier:
compressed
license:
copyright
format:
CSV
accesstypes:
download
accessType:
download
authentication:
none
authorization:
none
abbreviation:
ASCB
homePage: http://ascb.org/
ID:
SCR:010600
name:
American Society for Cell Biology
abbreviation:
CIL
homePage: http://www.cellimagelibrary.org/
ID:
SCR:003510
name:
Cell Image Library

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