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Title: Data from: The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways      
keywords:
Oct6
ChIP-Seq
RNA-Seq
Pluripotent stem cell
Neural commitment
type:
Article
description:
The neural fate commitment of pluripotent stem cells requires the repression of extrinsic inhibitory signals and the activation of intrinsic positive transcription factors. However, how these two events are integrated to ensure appropriate neural conversion remains unclear. Here, we showed that Pou3f1 is essential for the neural differentiation of mouse embryonic stem cells (ESCs), specifically during the transition from epiblast stem cells (EpiSCs) to neural progenitor cells (NPCs). Chimeric analysis showed that Pou3f1 knockdown leads to a markedly decreased incorporation of ESCs in the neuroectoderm. By contrast, Pou3f1-overexpressing ESC derivatives preferentially contribute to the neuroectoderm. Genome-wide ChIP-seq and RNA-seq analyses indicated that Pou3f1 is an upstream activator of neural lineage genes, and also is a repressor of BMP and Wnt signaling. Our results established that Pou3f1 promotes the neural fate commitment of pluripotent stem cells through a dual role, activating internal neural induction programs and antagonizing extrinsic neural inhibitory signals.
ID:
oai:datadryad.org:10255/dryad.58930
accesstypes:
download
landingPage: http://hdl.handle.net/10255/dryad.58930
authentication:
none
authorization:
none
ID:
doi:10.5061/dryad.3vk1g
Zhu Q, Song L, Peng G, Sun N, Chen J, Zhang T, Sheng N, Tang W, Qian C, Qiao Y, Tang K, Han J, Li J, Jing N (2014) The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways. eLife 3: e02224.
http://hdl.handle.net/10255/dryad.58930
elife-07-01-2014-RA-eLife-02224
setID:
hdl_10255_3
recNum:
28
setName:
Main
dateReleased:
07-16-2014
issueDate:
20140614
accessionDate:
06-19-2014
dateCreated:
06-19-2014
abbreviation:
Dryad
ID:
SCR:005910
name:
Dryad Digital Repository
homepage: http://www.datadryad.org
ID:
SCR:005910
name:
Dryad Data Repository

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