Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Data from: Transcriptional regulation of fruit ripening by tomato FRUITFULL homologs and associated MADS-box proteins      
keywords:
fruit ripening
tomato
MADS-box transcription factor
transcriptional regulation
type:
Article
description:
The tomato (Solanum lycopersicum) MADS box FRUITFULL homologs FUL1 and FUL2 act as key ripening regulators and interact with the master regulator MADS box protein RIPENING INHIBITOR (RIN). Here, we report the large-scale identification of direct targets of FUL1 and FUL2 by transcriptome analysis of FUL1/FUL2 suppressed fruits and chromatin immunoprecipitation coupled with microarray analysis (ChIP-chip) targeting tomato gene promoters. The ChIP-chip and transcriptome analysis identified FUL1/FUL2 target genes that contain at least one genomic region bound by FUL1 or FUL2 (regions that occur mainly in their promoters) and exhibit FUL1/FUL2-dependent expression during ripening. These analyses identified 860 direct FUL1 targets and 878 direct FUL2 targets; this set of genes includes both direct targets of RIN and nontargets of RIN. Functional classification of the FUL1/FUL2 targets revealed that these FUL homologs function in many biological processes via the regulation of ripening-related gene expression, both in cooperation with and independent of RIN. Our in vitro assay showed that the FUL homologs, RIN, and tomato AGAMOUS-LIKE1 form DNA binding complexes, suggesting that tetramer complexes of these MADS box proteins are mainly responsible for the regulation of ripening.
ID:
oai:datadryad.org:10255/dryad.57838
accesstypes:
download
landingPage: http://hdl.handle.net/10255/dryad.57838
authentication:
none
authorization:
none
ID:
doi:10.5061/dryad.4f7n5
Fujisawa M, Shima Y, Nakagawa H, Kitagawa M, Kimbara J, Nakano T, Kasumi T, Ito Y (2014) Transcriptional regulation of fruit ripening by tomato FRUITFULL homologs and associated MADS-box proteins. The Plant Cell 26(1): 89-101.
http://hdl.handle.net/10255/dryad.57838
setID:
hdl_10255_3
recNum:
27
setName:
Main
dateReleased:
03-27-2014
issueDate:
20140101
accessionDate:
03-27-2014
dateCreated:
03-27-2014
abbreviation:
Dryad
ID:
SCR:005910
name:
Dryad Digital Repository
homepage: http://www.datadryad.org
ID:
SCR:005910
name:
Dryad Data Repository

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.