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Title: Data from: Diagnostic gene expression biomarkers of coral thermal stress      
keywords:
cellular stress response
cellular homeostasis response
qPCR
double-gene assay
type:
Article
description:
Gene expression biomarkers can enable rapid assessment of physiological conditions in situ, providing a valuable tool for reef managers interested in linking organism physiology with large-scale climatic conditions. Here, we assessed the ability of quantitative PCR (qPCR) based gene expression biomarkers to evaluate (1) the immediate cellular stress response (CSR) of Porites astreoides to incremental thermal stress and (2) the magnitude of CSR and cellular homeostasis response (CHR) during a natural bleaching event. Expression levels largely scaled with treatment temperature, with the strongest responses occurring in heat shock proteins. This is the first demonstration of a “tiered” CSR in a coral, where the magnitude of expression change is proportional to stress intensity. Analysis of a natural bleaching event revealed no signature of an acute CSR in normal or bleached corals, indicating that the bleaching stressor(s) had abated by the day of sampling. Another long-term stress CHR-based indicator assay was significantly elevated in bleached corals; though assay values overall were low, suggesting good prospects for recovery. This study represents the first step in linking variation in gene expression biomarkers to stress tolerance and bleaching thresholds in situ by quantifying the severity of ongoing thermal stress and its accumulated long-term impacts.
ID:
oai:datadryad.org:10255/dryad.57706
accesstypes:
download
landingPage: http://hdl.handle.net/10255/dryad.57706
authentication:
none
authorization:
none
ID:
doi:10.5061/dryad.1h0n1
Kenkel CD, Sheridan C, Leal MC, Bhagooli R, Castillo KD, Kurata N, McGinty E, Goulet TL, Matz MV (2013) Diagnostic gene expression biomarkers of coral thermal stress. Molecular Ecology Resources 14(4): 667–678.
http://hdl.handle.net/10255/dryad.57706
molecolres-MER-13-0362
setID:
hdl_10255_3
recNum:
19
setName:
Main
dateReleased:
06-16-2014
issueDate:
20140110
accessionDate:
12-17-2013
dateCreated:
12-17-2013
abbreviation:
Dryad
ID:
SCR:005910
name:
Dryad Digital Repository
homepage: http://www.datadryad.org
ID:
SCR:005910
name:
Dryad Data Repository

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