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Title: Data from: Down-regulation of Rad51 activity during meiosis in yeast prevents competition with Dmc1 for repair of double-strand breaks      
keywords:
meiosis
Dmc1
Rad51
recombination
interhomolog
intersister
yeast
type:
Article
description:
Interhomolog recombination plays a critical role in promoting proper meiotic chromosome segregation but a mechanistic understanding of this process is far from complete. In vegetative cells, Rad51 is a highly conserved recombinase that exhibits a preference for repairing double strand breaks (DSBs) using sister chromatids, in contrast to the conserved, meiosis-specific recombinase, Dmc1, which preferentially repairs programmed DSBs using homologs. Despite the different preferences for repair templates, both Rad51 and Dmc1 are required for interhomolog recombination during meiosis. This paradox has recently been explained by the finding that Rad51 protein, but not its strand exchange activity, promotes Dmc1 function in budding yeast. Rad51 activity is inhibited in dmc1Δ mutants, where the failure to repair meiotic DSBs triggers the meiotic recombination checkpoint, resulting in prophase arrest. The question remains whether inhibition of Rad51 activity is important during wild-type meiosis, or whether inactivation of Rad51 occurs only as a result of the absence of DMC1 or checkpoint activation. This work shows that strains in which mechanisms that down-regulate Rad51 activity are removed exhibit reduced numbers of interhomolog crossovers and noncrossovers. A hypomorphic mutant, dmc1-T159A, makes less stable presynaptic filaments but is still able to mediate strand exchange and interact with accessory factors. Combining dmc1-T159A with up-regulated Rad51 activity reduces interhomolog recombination and spore viability, while increasing intersister joint molecule formation. These results support the idea that down-regulation of Rad51 activity is important during meiosis to prevent Rad51 from competing with Dmc1 for repair of meiotic DSBs.
ID:
oai:datadryad.org:10255/dryad.55748
accesstypes:
download
landingPage: http://hdl.handle.net/10255/dryad.55748
authentication:
none
authorization:
none
ID:
doi:10.5061/dryad.8gh60
Liu Y, Gaines WA, Callender T, Busygina V, Oke A, Sung P, Fung JC, Hollingsworth NM (2014) Down-regulation of Rad51 activity during meiosis in yeast prevents competition with Dmc1 for repair of double-strand breaks. PLoS Genetics 10(1): e1004005.
http://hdl.handle.net/10255/dryad.55748
pgenetics-PGENETICS-D-13-01530R2
setID:
hdl_10255_3
recNum:
75
setName:
Main
dateReleased:
01-28-2014
issueDate:
20140123
accessionDate:
01-28-2014
dateCreated:
01-28-2014
abbreviation:
Dryad
ID:
SCR:005910
name:
Dryad Digital Repository
homepage: http://www.datadryad.org
ID:
SCR:005910
name:
Dryad Data Repository

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