Other

PRJNA339793

Translation of poly(A) tails leads to mRNA cleavage but the mechanism and global pervasiveness of this “nonstop/no-go” decay process is not understood. Here we performed ribosome profiling of short 15-18 nt mRNA footprints to identify ribosomes stalled at 3’ ends of mRNA decay intermediates. We found mRNA cleavage extending hundreds of nucleotides upstream of ribosome stalling in poly(A) and predominantly in one reading frame. These observations suggest that cleavage is closely associated with the ribosome. Surprisingly, ribosomes appeared to stall when as few as 3 consecutive ORF-internal lysine codons were positioned in the A, P, and E sites though significant mRNA degradation was not observed. Endonucleolytic cleavage was widespread, however, at sites of premature polyadenylation and rescue of the ribosomes stalled at these sites was dependent on Dom34. These results suggest this process may be critical when changes in polyadenylation occur during development, tumorigenesis, or when translation termination/recycling is impaired. Overall design: 14 samples are included in the study (2 mRNA-Seq samples and 12 ribosome footprinting samples). Read sizes were examined for lengths of 15-18 nt or 25-34 nt or both in some cases, reads were aligned by 5' or 3' ends or both in some cases.

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pmid:28193672

Saccharomyces cerevisiae

ncbitax:4932

NCBI

SCR:006472

National Center for Biotechnology Information

SCR:004801

NCBI BioProject