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Title: Translation of poly(A) tails leads to precise mRNA cleavage and widespread ribosome rescue      
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Other
ID:
PRJNA339793
description:
Translation of poly(A) tails leads to mRNA cleavage but the mechanism and global pervasiveness of this “nonstop/no-go” decay process is not understood. Here we performed ribosome profiling of short 15-18 nt mRNA footprints to identify ribosomes stalled at 3’ ends of mRNA decay intermediates. We found mRNA cleavage extending hundreds of nucleotides upstream of ribosome stalling in poly(A) and predominantly in one reading frame. These observations suggest that cleavage is closely associated with the ribosome. Surprisingly, ribosomes appeared to stall when as few as 3 consecutive ORF-internal lysine codons were positioned in the A, P, and E sites though significant mRNA degradation was not observed. Endonucleolytic cleavage was widespread, however, at sites of premature polyadenylation and rescue of the ribosomes stalled at these sites was dependent on Dom34. These results suggest this process may be critical when changes in polyadenylation occur during development, tumorigenesis, or when translation termination/recycling is impaired. Overall design: 14 samples are included in the study (2 mRNA-Seq samples and 12 ribosome footprinting samples). Read sizes were examined for lengths of 15-18 nt or 25-34 nt or both in some cases, reads were aligned by 5' or 3' ends or both in some cases.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA339793
authentication:
none
authorization:
none
ID:
pmid:28193672
name:
Saccharomyces cerevisiae
ncbiID:
ncbitax:4932
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject