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Title: Human immunodeficiency virus 1 : HIV-1 Envelope Clinical Isolates from Vicriviroc Resistant Subjects      
keywords:
raw sequence reads
ID:
PRJNA317236
description:
The HIV-1 envelope interacts with coreceptors CCR5 and CXCR4 in a dynamic, multi-step process, its molecular details not clearly delineated. Clinical application of CCR5 antagonists often results in tropism shift and subsequent therapeutic failure. Here we describe a novel experimental approach using full-length patient-derived gp160 quasispecies libraries cloned into HIV-1 molecular clones, separation of these quasispecies based on their phenotypic tropism in vitro, and deep sequence of the resultant functional variants by Illumina HiSeq and Pacific Biosciences for structure-function analyses. Through in-depth analysis of functionally validated envelope sequences from patients who failed CCR5 antagonist therapy, we revealed determinants strongly associated with coreceptor specificity.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA317236
authentication:
none
authorization:
none
name:
Human immunodeficiency virus 1
ncbiID:
ncbitax:11676
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject