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Title: RNA-Seq analysis of the pancreatic islets of beta cell specific adult Nkx2.2 mutant mice      
keywords:
Transcriptome or Gene expression
ID:
PRJNA316851
description:
Aim:Transcriptional analysis of the pancreatic islets of adult Nkx2.2 flox/flox; RipCre mice versus control Methods:Pancreatic islets from 4week old Nkx2.2 mutant mice and controls were isolated and total RNA was extracted.Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 20 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq. Results: Among the downregulated genes with a p-value=0.05 are important genes for beta cell function and idenity.Among the upregulated genes with a p-value=0.05 are non beta endocrine hormones. Conclusion: Nkx2.2 activates important beta cell genes and actively represses non beta cell genes Overall design: mRNA profiles of the pancreatic islets of 4 week old control and Nkx2.2 mutant mice were generated by deep sequencing , in triplicate, using Illumina HiSeq2000.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA316851
authentication:
none
authorization:
none
ID:
pmid:27941248
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • P30 CA016087/CA/NCI NIH HHS/United States

  • P30 CA015704/CA/NCI NIH HHS/United States

  • P30 DK020541/DK/NIDDK NIH HHS/United States

  • R01 DK082590/DK/NIDDK NIH HHS/United States

  • R01 DK088383/DK/NIDDK NIH HHS/United States

  • P30 DK063608/DK/NIDDK NIH HHS/United States

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