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Title: Expression data from consecutive stages of human early in vitro T-cell differentiation      
keywords:
Transcriptome or Gene expression
ID:
PRJNA315664
description:
Human T-cell development is less well studied than its murine counterpart due to the lack of genetic tools and the difficulty of obtaining cells and tissues. However, recent technological advances allow identification of the transcriptional landscape of differentiating human thymocytes. Here we report the gene expression profiles of 11 immature, consecutive T-cell developmental stages. The changes in gene expression of cultured stem cells on OP9-DL1 match those of ex vivo isolated human thymocytes. These analyses led us to define evolutionary conserved gene signatures that represent pre- and post- αβ T-cell commitment stages. We found that loss of CD44 marks T-cell commitment in early CD7+CD5+CD45dim cells, before the acquisition of CD1a surface expression. The CD44-CD1a- post-committed thymocytes have initiated in frame TCR rearrangements and have completely lost the capacity to develop into myeloid, B- and NK-cells, unlike uncommitted CD44+CD1a- thymocytes. Therefore, loss of CD44 represents a previously unrecognized stage that defines the earliest committed T-cell population in the human thymus. We used microarrays to detail the transcriptional program underlying human early T-cell differentiation, from CD34+ HSCs until the CD4+CD8+ DP stage. Overall design: Throughout the differentiation of human HSC towards the T-cell lineage, we sorted T-cell fractions at 5 time points (days 0, 7, 12, 18, and 27) based on the expression of cell surface markers including CD45, CD34, CD7, CD5, CD1a, CD4 and CD8. Following the sorting of consecutive early T-cell development stages, the samples were then used for RNA extraction and hybridization on Affymetrix microarrays.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA315664
authentication:
none
authorization:
none
ID:
pmid:28163708
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject