Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Prp4 Kinase Grants the License to Splice: Control of Weak Splice Sites during Spliceosome Activation      
keywords:
Other
ID:
PRJNA304451
description:
The genome of the fission yeast Schizosaccharomyces pombe encodes 17 kinases that are essential for cell growth. These include the cell-cycle regulator Cdc2, as well as several kinases that coordinate cell growth, polarity, and morphogenesis during the cell cycle. In this study, we further characterized another of these essential kinases, Prp4, and showed that the splicing of many introns is dependent on Prp4 kinase activity. For detailed characterization, we chose the genes res1 and ppk8, each of which contains one intron of typical size and position. Splicing of the res1 intron was dependent on Prp4 kinase activity, whereas splicing of the ppk8 intron was not. Extensive mutational analyses of the 5’ splice site of both genes revealed that proper transient interaction with the 5’ end of snRNA U1 governs the dependence of splicing on Prp4 kinase activity. Proper transient interaction between the branch sequence and snRNA U2 was also important. Therefore, the Prp4 kinase is required for recognition and efficient splicing of introns displaying weak exon1/5’ splice sites and weak branch sequences. Overall design: RNAseq was performed on three samples in which a conditional mutant was analyzed in three time point (time 0, 30 and 60 minutes).
accesstypes:
download
landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA304451
authentication:
none
authorization:
none
name:
Schizosaccharomyces pombe
ncbiID:
ncbitax:4896
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject