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Title: RNA seq of intracellular L. monocytogenes : A detailed view of the intracellular transcriptome of Listeria monocytogenes in murine macrophages using RNA-seq      
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ID:
PRJEB10393
description:
Listeria monocytogenes is a bacterial pathogen and causative agent for the foodborne infection listeriosis, which is especially a threat for pregnant, elderly or immunocompromised humansindividuals. Due to its ability to invade and colonize diverse eukaryotice cell types including cells from invertebrates, L. monocytogenes has become a well-established model organism for intracellular growth. Nearly Almost ten years ago, we and others presented the first whole-genome microarray-based intracellular transcriptome of this bacteriumL. monocytogenes. With the advent of newer technologies to addressing transcriptomes in greater detail, we revisit this work, and analyze the intracellular transcriptome of L. monocytogenes during growth in murine macrophages using a deep sequencing based approach. We detected 656 differentially expressed genes of which 367 were upregulated. We found that ~64% of all regulated genes previously obtained fromidentified by microarray analysis could be confirmed in this study. Many of the additional genes that were detected in the currentis study involve transporters for various metals, ions as well as complex sugars such as mannose. This study also afforded allowed us with an opportunity to detect and map antisense transcripts, especially those upregulated during intracellular bacterial growth. Another particular finding was the detection of regulatory changes for a subset of temperate A118-like prophage genes, thereby providing insights shedding light on into the transcriptional profile of this bacteriophage during intracellular growth. In total, Tthe study provides an updated genome-wide view of the transcriptional landscape of the bacteriaL. monocytogenes during intracellular growth and provides represents a rich resource for further detailed analysis.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJEB10393
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authorization:
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dateReleased:
10-17-2015
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject