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Title: Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics      
keywords:
Transcriptome or Gene expression
ID:
PRJNA298916
description:
Consumption of a high fat meal can increase neutrophilic airway inflammation in asthma. This study investigates the molecular mechanisms driving airway neutrophilia following a high fat meal in asthma. Overall design: a subset of subjects (asthmatics, n=5; healthy controls, n=3) were examined by microarray. A total of 500 ng of sputum RNA was amplified and labelled with biotin-uridine triphosphate using the Total Prep RNA amplification kit as per manufacturer’s instructions (Life Technologies). 750 ng cRNA was hybridised to Illumina Sentrix HumanRef-8 V3 Expression BeadChips (Illumina, San Diego, CA, USA) using standard protocols. Each BeadChip measured the expression of 24,354 genes and was scanned using the Illumina Bead Station and captured using BeadScan 3.5.11 (Illumina)
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA298916
authentication:
none
authorization:
none
ID:
pmid:26751474
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject