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Title: Next Generation Sequencing Facilitates Quantitative Analysis of chromatin loci bound by DDX5 in cultured T helper 17 cell [ChIP-Seq]      
keywords:
Epigenomics
ID:
PRJNA298542
description:
Purpose: The goals of this study are to identify chromatin loci bound by DDX5 in cultured T helper 17 cell in wildtype and DDX5 deficient background. Methods: ChIP-seq profiles of 48hrs in vitro cultured T helper 17 cells from wild-type mice were generated by deep sequencing, using Illumina HighSeq. The sequence reads that passed quality filters were mapped by Bowtie2.0. Results: DDX5 were found to be enriched on a subset of previously characterized RORgt occupied sites on the chromatin. Conclusions: Our study suggest that a subset of RORgt occupied regions were co-bound by DDX5. Overall design: DDX5-associated-DNA profiles of 48hr in vitro cultured Th17 from wild type (WT) and DDX5 T cell specific conditional knockout mice were generated by deep sequencing using Illumina HighSeq
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA298542
authentication:
none
authorization:
none
ID:
pmid:26675721
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • T32 CA009161/CA/NCI NIH HHS/United States

  • P30 CA016087/CA/NCI NIH HHS/United States

  • R01AI080885/AI/NIAID NIH HHS/United States

  • R01HG004361/HG/NHGRI NIH HHS/United States

  • P50-HG007735/HG/NHGRI NIH HHS/United States

  • R01DK103358/DK/NIDDK NIH HHS/United States

  • R01 AI121436/AI/NIAID NIH HHS/United States

  • R01 DK101520/DK/NIDDK NIH HHS/United States

  • Howard Hughes Medical Institute/United States

  • 1F30CA189514-01/CA/NCI NIH HHS/United States

  • T32 AI100853/AI/NIAID NIH HHS/United States

  • F30 CA189514/CA/NCI NIH HHS/United States

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