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Title: Identification of a gene cluster involved in the utilization of phenylpropanoids in Corynebacterium glutamicum [set1]      
keywords:
Transcriptome or Gene expression
ID:
PRJNA285457
description:
DNA microarray experiments with phenylpropanoid pulses were performed in order to get hints on expression of relevant genes or gene clusters activated in presence of phenylpropanoid compounds. We determined the relative mRNA-levels of wild-type cells confronted with phenylpropanoids and unpulsed cells. A pulsing time of 30 minutes was sufficient to find regulation of gene expression in response to the phenylpropanoid amount of 5 mM (final concentration) added. A relatively short pulsing time was preferred over the cultivation of the strain with phenylpropanoids in comparison to growth on glucose because a completely different growth behavior was expected when using two completely unrelated carbon sources. Overall design: DNA microarray experiments were performed in two biological replicates. The transcriptome of C. glutamicum cells pulsed for 30 minutes before harvest with 5 mM of phenylpropanoids (p-coumaric acid) was compared to the transcriptome of C. glutamicum wt cells.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA285457
authentication:
none
authorization:
none
ID:
pmid:26610800
name:
Corynebacterium glutamicum
ncbiID:
ncbitax:1718
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject