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Title: Bacteria differently regulate mRNA abundance to specifically respond to various stresses      
keywords:
Transcriptome or Gene expression
ID:
PRJNA283800
description:
Environmental stress is detrimental to cell viability and requires an adequate reprogramming of cellular activities to maximize cell survival. We present a global analysis of the response of Escherichia coli to acute heat and osmotic stress. We combine deep sequencing of total mRNA and ribosome-protected fragments to provide a genome-wide map of the stress response at transcriptional and translational level. For each type of stress, we observe a unique subset of genes that shape the stress-specific response. Upon temperature upshift, mRNAs with reduced folding stability up- and downstream of the start codon, and thus with more accessible initiation regions, are translationally favoured. Conversely, osmotic upshift causes a global reduction of highly-translated transcripts with high copy numbers, allowing reallocation of translation resources to undegraded and newly synthesised mRNAs Overall design: Comparing global transcriptional and translational control by mRNA-Seq and Ribosome Profiling (mRNA-Seq of ribosome protected fragments – RPF)
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA283800
authentication:
none
authorization:
none
name:
Escherichia coli str. K-12 substr. MC4100
ncbiID:
ncbitax:1403831
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject