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Title: Gene Expression Comparing Col to WS to Identify conserved cold-responsive Affymetrix signatures      
keywords:
Transcriptome or Gene expression
ID:
PRJNA271353
description:
Gene expression Arabidopsis 24h cold-treated, 4c, seedlings to identify a *gold-standard* set of cold-responsive transcripts. Most of the CBF overexpression lines are in WS, therefore, it is necessary to identify a consistent set of transcripts that are detectible as cold-induced on the ATH1 platform for both WS and Columbia, so that appropriate comparisons can be made to determine the effects of low temperature in CBF overexpressing or loss of function plants in a WS background and an attempt can be made to compare the results of altered CBF function to published microarray studies. We aimed to identify a set of *gold-standard* cold responsive transcripts that were induced in multiple different experiments performed by different researchers and were detectible in both the WS and Col-0 ecotypes. This series contributes the 24h cold-treated WS ecotypes, along with additional Col-0 cold-treated and control samples for normalization purposes. Keywords: Expression profiling by array Overall design: Col-0 and WS seedlings were sown on Gamborg's media with 0.7% agar, stratified for 3d, and grown in continuous low light (60-80 microE) at 22C, for 10 days and either moved to a new 22C chamber or transferred to a 4C chamber. After 24h tissue was harvested and frozen in liquid nitrogen. Whole seedlings (including root tissue) were taken from the plate).
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA271353
authentication:
none
authorization:
none
name:
Arabidopsis thaliana
ncbiID:
ncbitax:3702
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject