Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Functional screen for novel regulators of murine hematopoietic stem cell in vivo repopulating activity      
keywords:
Transcriptome or Gene expression
ID:
PRJNA269178
description:
Although Hematopoietic Stem Cell Transplantation (HSCT) routinely treats hematologic disease, many patients experience adverse outcomes. Understanding the molecular regulation of HSC engraftment is paramount to improving HSCT regimens. Here, we executed a large-scale transplant-based functional screen for novel regulators of HSC repopulation.. Of >50 gene candidates tested, 18 were required for in vivo hematopoietic repopulation and two were detrimental to repopulation, as their loss enhanced this activity. Each Hit was validated in a second screen. Eleven Hits have never before been implicated in HSC biology. We further show that one novel Hit, Foxa3, is required for optimal engraftment as Foxa3-/- bone marrow is defective in both primary and secondary hematopoietic reconstitution. We also present evidence that Foxa3 is a novel pioneer factor in HSC. Each gene identified in our screen is a window into the cellular mechanisms that control hematopoietic reconstitution. Thus, this work represents a resource to the community to better understand these processes Overall design: 3 FOXA3 KO samples are compared to 3 wt samples
accesstypes:
download
landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA269178
authentication:
none
authorization:
none
ID:
pmid:26880577
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • R01 DK104028/DK/NIDDK NIH HHS/United States

  • R01 GM104369/GM/NIGMS NIH HHS/United States

  • K01DK080846/DK/NIDDK NIH HHS/United States

  • P30 CA021765/CA/NCI NIH HHS/United States

  • R01 HG006130/HG/NHGRI NIH HHS/United States

  • R21 HD081054/HD/NICHD NIH HHS/United States

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.