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Title: HuR- dependent regulation of mRNA splicing is essential for the B cell antibody response [Ribo-Seq]      
keywords:
Other
ID:
PRJNA263261
description:
Post-transcriptional regulation of mRNA by the RNA binding protein HuR is required in B cells for the germinal centre reaction and for the production of class-switched antibodies in response to T-independent antigens. Transcriptome-wide examination of RNA isoforms, abundance and translation in HuR-deficient B cells, together with direct measurements of HuR-RNA interaction, revealed that HuR-dependent mRNA splicing affects hundreds of transcripts including the dihydrolipoyl succinyltransferase (Dlst), a subunit of the aketoglutaratedehydrogenase (aKGDH) enzyme. In the absence of HuR, defective mitochondrial metabolism results in high levels of reactive oxygen species and B cell death. Our study shows how post-transcriptional processes control the balance of energy metabolism required for B cell proliferation and differentiation. Overall design: Sequencing analysis of ribosome protected RNA fragments in ex vivo or LPS-activated splenic B cells was performed using ARTseq™ Ribosome Profiling Kit - Mammalian (Epicentre, RPHMR12126) and Illumina platform. Splenic B cells come from HuRf/f control or HuR cKO mice. 4-5 biological replicates per genotype and condition.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA263261
authentication:
none
authorization:
none
ID:
pmid:25706746
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • BBS/E/B/000C0409/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom

  • BB/J001457/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom

  • BB/L009986/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom

  • P01 AG014930/AG/NIA NIH HHS/United States

  • BBS/E/B/000C0407/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom

  • BB/J00152X/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom

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