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Title: Next generation sequencing of Phaeodactylum tricornutum grown with and without nitrogen      
keywords:
Transcriptome or Gene expression
ID:
PRJNA242990
description:
We have done next generation sequencing of optically thin, exponentialy growing Phaeodactylum tricornutum cultures grown with and without nitrogen source to improve our undestanding of the pathways regulation under conditions that promote lipid accumulation (N-starvation). Overall design: 3 biologically independent exponentially growing culture of Phaeodactylum tricornutum were pelleted and washed several times with N-free media. Each culture was devided to 2 replicates with initial cell concentration of 2X105 cells/mL, one with NaNO3 as nitrogen source and the other without any nitrogen source. The cuture were bubbled foro 48 hours and sampled for transcriptome together with other physiological parameters and lipid analysis.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA242990
authentication:
none
authorization:
none
ID:
pmid:25548193
name:
Phaeodactylum tricornutum
ncbiID:
ncbitax:2850
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject