biomedical and healthCAre Data Discovery Index Ecosystem
biomedical and healthCAre Data Discovery Index Ecosystem
| Title: | Genome-wide analysis of gene expression and intron retention during development in a U12-type splicing-deficient zebrafish mutant [RNA-seq]
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| keywords: |
Transcriptome or Gene expression
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| ID: |
PRJNA236557
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| description: |
To determine the global impact of the clbn mutation on gene expression and efficiency of U2- and U12-type splicing, we analyzed the transcriptome of 108hpf wt and clbn mutant larvae by microarrays and RNA sequencing. RNAseq data was used to characterize intron retention of U2-type and U12-type intron on a genome-wide scale to confirm that rnpc3 deficiency specifically impairs U12-type splicing. RNAseq and microarray data were combined to yield high-confidence lists of differentially expressed genes which show that impaired U12-type splicing has a wide-ranging effect on the developing transcriptome.
Overall design: RNAseq libraries prepared from 108 hours post-fertilization zebrafish larvae (approx. 60 embryos each, genotyped homozygous wildtype and homozygous clbns841 mutants, respectively)
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| accesstypes: |
download
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| landingpage: | http://www.ncbi.nlm.nih.gov/bioproject/PRJNA236557 |
| authentication: |
none
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| authorization: |
none
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| ID: |
pmid:24516132
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| name: |
Danio rerio
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| ncbiID: |
ncbitax:7955
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| abbreviation: |
NCBI
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| homePage: | http://www.ncbi.nlm.nih.gov |
| ID: |
SCR:006472
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| name: |
National Center for Biotechnology Information
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| homePage: | http://www.ncbi.nlm.nih.gov/bioproject |
| ID: |
SCR:004801
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| name: |
NCBI BioProject
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