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Title: Comparative total RNA and mRNA sequencing and systems analysis reveals nascent transcriptional response to early HIV-1 infection in a CD4+ T cell line      
keywords:
Transcriptome or Gene expression
ID:
PRJNA234297
description:
To systematically investigate early host transcriptional response to HIV-1 infection, including polyadenylated and non-polyadenylated transcripts, we separately sequenced Total RNAs (Total RNA-seq) from HIV-1-infected SupT1 cells, a human CD4+ T cell line. We show many non-polyadenylated RNAs were also differentially expressed upon HIV-1 infection, as expected only detected by Total RNA-seq. Interestingly, we identified 8 times more differentially expressed genes at 12 hour post-infection by Total RNA-seq than by mRNA-seq, mostly protein-coding genes. Overall design: 6 Total RNA sample sets were sequenced. Samples were either Mock infected, HIV-1 infected, or UV-inactivated viron infected for either 12 hours or 24 hours. Each sample set contains replicates. mRNA-seq data was processed previously and is part of a different study. It was referenced in this study. The mRNA-seq data can be found in GSE38006. This data set refers to the Whole Transcriptome data from similar samples.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA234297
authentication:
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authorization:
none
ID:
pmid:24850744
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject