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Title: Knock-down of BCL6 expression in human Diffuse Large B-Cell Lymphoma cell lines      
keywords:
Transcriptome or Gene expression
ID:
PRJNA196519
description:
This dataset was used to benchmark the Virtual Inference of Protein-activity by Regulon Readout algorithm (VIPER). Despite recent advances in molecular profiling, proteome-wide assessment of protein activity in individual samples remains a highly elusive target. In stark contrast, protein activity quantitation is increasingly critical to the dissection of key regulatory processes and to the elucidation of biologically relevant mechanisms. Importantly, its value extends to the study of drug activity, as most small molecules inhibit activity of their cognate protein substrates without affecting the protein’s or associated mRNA’s abundance. VIPER leverages the increasingly accurate and context specific knowledge of regulatory networks. Specifically, it uses the expression of the transcriptional targets most directly regulated by a given protein in an individual sample as a reporter for the computational inference of its activity. Overall design: BCL6 knock down experiments were performed in OCI-Ly7 and Pfeiffer GCB-DLBCL cell lines. Both cell lines were maintained in 10% FBS supplemented IMDM medium and transiently transfected with either a BCL6-specific or a non-target control siRNA oligo in triplicate. Total RNA was isolated 48h after transfection and gene expression was profiled on H-GU133plus2 Affymetrix gene chips.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA196519
authentication:
none
authorization:
none
ID:
pmid:27322546
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • U54 CA121852/CA/NCI NIH HHS/United States

  • U01 CA168426/CA/NCI NIH HHS/United States

  • S10 OD012351/OD/NIH HHS/United States

  • R01 CA085573/CA/NCI NIH HHS/United States

  • U01 CA164184/CA/NCI NIH HHS/United States

  • S10 OD021764/OD/NIH HHS/United States

  • P30 CA013330/CA/NCI NIH HHS/United States

  • R35 CA197745/CA/NCI NIH HHS/United States

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