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Title: Alanine scan of 11 amino acid long novel linear epitope from C. jejuni cj0669 protein      
keywords:
Proteome
ID:
PRJNA194602
description:
A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168, which was previously identified as potentially immunogenic, was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence. Overall design: Twelve peptides, one representing the original sequence and eleven peptides with each residue replaced by alanine in turn, were synthesized on microarrays by JPTs Pepstar Technology. For each microarray, nine replicates for each peptide were spotted. The microarray was separated into three incubation chambers by the ProPlate 3-well module (Grace Biolabs) to allow for incubation with different antibodies in parallel. For specific interactions, rabbit polyclonal IgG to C. jejuni was used, while unspecific binding to the epitope sequence was checked using rabbit polyclonal IgG to Salmonella enterica.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA194602
authentication:
none
authorization:
none
ID:
pmid:23734261
name:
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
ncbiID:
ncbitax:192222
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject