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Title: Distinct roles for IL-27 and IFN-g in the development of T-bet+ Treg required to limit infection-induced pathology      
keywords:
Transcriptome or Gene expression
ID:
PRJNA168438
description:
IL-27 is a potent antagonist of TH1-mediated inflammation, but the basis for this effect is not fully understood. Recent studies identified a population of T-bet+ CXCR3+ Treg that limit TH1-mediated immune pathology. The studies presented here demonstrate that IL-27-mediated STAT1 activation promotes Treg expression of T-bet and CXCR3. Infection with Toxoplasma gondii induced a similar Treg population that limits T cell responses and this population at mucosal sites is IL-27-dependent. Furthermore, transfer of Treg ameliorated the infection-induced CD4+ T cell-mediated pathology observed in IL-27p28-/- mice. Although IFN-γ promoted a similar population of cells in the periphery, it did not compensate for the absence of IL-27 at mucosal sites and microarray analysis revealed that Treg exposed to either cytokine have distinct transcriptional profiles. These findings suggest that IFN-γ and IL-27 have different roles in Treg biology but define IL-27 as a key cytokine that promotes the development of Treg specialized to control TH1 immunity. Overall design: Three conditions were analyzed across two timepoints. Inducible regulatory T cells (iTreg) were generated in vitro in the presence of IL-27, IFNg or under 'Neutral' conditions as a control. Samples were collected at 10 hours and 2 days during the culture period. Three biological replicates were used for each condition.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA168438
authentication:
none
authorization:
none
ID:
pmid:22981537
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • AI084882/AI/NIAID NIH HHS/United States

  • R01 AI042334/AI/NIAID NIH HHS/United States

  • R37-AI28724/AI/NIAID NIH HHS/United States

  • T32 AI055428/AI/NIAID NIH HHS/United States

  • T32 AI007532/AI/NIAID NIH HHS/United States

  • AI 071302/AI/NIAID NIH HHS/United States

  • R21-AI090234-01/AI/NIAID NIH HHS/United States

  • P01 AI043620/AI/NIAID NIH HHS/United States

  • R21 AI090234/AI/NIAID NIH HHS/United States

  • R01 AI061699/AI/NIAID NIH HHS/United States

  • T32 AI055400/AI/NIAID NIH HHS/United States

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