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Title: Comparison of gene expression patterns between Lgr5 positive cells in adenomas and small intestine      
keywords:
Transcriptome or Gene expression
ID:
PRJNA165691
description:
The generation of the Lgr5_EGFP_ires_CreERT2 knock-in mouse allows marking of Lgr5 positive cells of different tissues by GFP expression. Here we use these mice to sort GFP positive cells from intestinal adenomas and compare those to GFP positive cells from normal small intestine. Overall design: In Lgr5_EGFP_ires_CreERT2 knock-in mice, GFP is expressed under the control of the Lgr5 promoter, leading to high GFP levels in Lgr5 positive cells (GFP-high). In normal small intestine, GFP-high cells mark crypt base columnar cells, the stem cells of small intestine. Intestinal adenomas can be generated in these mice when crossed to Apc_fl/fl mice by inducing Apc deletion through the Lgr5 driven Cre. We isolated GFP-high cells both from intestinal adenomas and normal small intestine of Lgr5-ki mice without the Apc_fl/fl allele and compared them to each other on 4X44K Agilent Whole Mouse Genome dual colour Microarrays (G4122F). Three independent sorts were performed and RNA of GFP-high adenoma cells hybridized directly against RNA of GFP-high cells from normal small intestine in three dye swap experiments, resulting in six individual arrays.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA165691
authentication:
none
authorization:
none
ID:
pmid:22855427
dateReleased:
08-13-2012
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject