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Title: The Conserved Polarity Factor PodJ1 Impacts Multiple Cell Envelope-Associated Functions in Sinorhizobium meliloti      
keywords:
Transcriptome or Gene expression
ID:
PRJNA146015
description:
Although diminutive in size, bacteria possess highly diverse and spatially confined cellular structures. Two related alpha-proteobacteria, Sinorhizobium meliloti and Caulobacter crescentus, serve as models for investigating the genetic basis of morphologic variations. S. meliloti, a symbiont of leguminous plants, synthesizes multiple flagella and no prosthecae, whereas C. crescentus, a freshwater bacterium, has a single polar flagellum and stalk. The podJ gene, originally identified in C. crescentus for its role in polar organelle development, is split into two juxtaposed open reading frames, podJ1 and podJ2, in S. meliloti. Deletion of podJ1 interferes with flagellar motility, exopolysaccharide production, cell envelope integrity, cell division, and normal morphology, but not symbiosis. As in C. crescentus, the S. meliloti PodJ1 protein appears to act as a polarity beacon and localizes to the newer cell pole. Microarray analysis indicates that podJ1 affects the expression of at least 129 genes, the majority of which correspond to observed mutant phenotypes. The combination of phenotypic characterization, microarray analysis, and suppressor identification suggests that PodJ1 controls a core set of conserved elements, including flagellar and pili genes, the signaling proteins PleC and DivK, and the TacA transcriptional activator, while alternate downstream targets have evolved to suit the distinct lifestyles of individual species. Overall design: Gene expression profiling of Sinorhizobium meliloti Rm1021 or its isogenic podJ1 deletion mutant, grown to mid exponential phase in rich medium, was performed using custom Affymetrix GeneChips.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA146015
authentication:
none
authorization:
none
ID:
pmid:22553970
name:
Sinorhizobium meliloti
ncbiID:
ncbitax:382
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • R01-GM093628/GM/NIGMS NIH HHS/United States

  • SC2 GM082318-03/GM/NIGMS NIH HHS/United States

  • F32 GM067472/GM/NIGMS NIH HHS/United States

  • SC2 GM082318/GM/NIGMS NIH HHS/United States

  • R25 GM048972/GM/NIGMS NIH HHS/United States

  • SC2 GM082318-02/GM/NIGMS NIH HHS/United States

  • R25 GM059298/GM/NIGMS NIH HHS/United States

  • SC2 GM082318-01/GM/NIGMS NIH HHS/United States

  • SC2 GM082318-03S1/GM/NIGMS NIH HHS/United States

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