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Title: Androgen Receptor Gene Expression in Prostate Cancer is Directly Suppressed by the Androgen Receptor Through Recruitment of Lysine Specific Demethylase 1      
keywords:
Transcriptome or Gene expression
ID:
PRJNA145741
description:
Androgen receptor (AR) is reactivated in castration resistant prostate cancer (CRPC) through mechanisms including marked increases in AR gene expression. We identify an enhancer in the AR second intron contributing to increased AR expression at low androgen levels in CRPC. Moreover, at increased androgen levels the AR binds this site and represses AR gene expression through recruitment of lysine specific demethylase 1 (LSD1) and H3K4me1,2 demethylation. AR similarly represses expression of multiple genes mediating androgen synthesis, DNA synthesis and proliferation, while stimulating genes mediating lipid and protein biosynthesis. Androgen levels in CRPC appear adequate to stimulate AR activity on enhancer elements, but not on suppressor elements, resulting in increased expression of AR and AR repressed genes that contribute to cellular proliferation. Overall design: Custom Agilent 44K whole human genome expression oligonucleotide microarrays were used to profile pre-castrated androgen dependent, 4d-post-castrated, and relapsed castration resistant VCaP xenograft tumors in 3 mice. Total RNA was isolated and amplified prior to hybridization against a common reference pool of prostate tumor cell lines.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA145741
authentication:
none
authorization:
none
ID:
pmid:22014572
dateReleased:
08-16-2011
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • P50 CA090381/CA/NCI NIH HHS/United States

  • RC1 CA146849/CA/NCI NIH HHS/United States

  • P50 CA097186/CA/NCI NIH HHS/United States

  • P50 CA090381-06/CA/NCI NIH HHS/United States

  • R00 CA135592/CA/NCI NIH HHS/United States

  • R01 CA111803/CA/NCI NIH HHS/United States

  • R01 CA111803-03/CA/NCI NIH HHS/United States

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