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Title: The Regulatory Specificity of a Homeodomain Protein is Determined by Unique DNA-Binding Sequences      
keywords:
Transcriptome or Gene expression
ID:
PRJNA137407
description:
Homeodomain (HD) proteins comprise a large family of evolutionarily conserved transcription factors (TFs) having diverse developmental functions, yet they paradoxically recognize very similar DNA sequences. To investigate how HDs control cell-specific gene expression patterns, we determined the DNA binding specificities of a broad range of HDs critical for Drosophila embryonic mesoderm development. These studies revealed particular sequences that are bound by one HD and not by others. Such HD-preferred binding sites are overrepresented in the noncoding regions of genes that are regulated by the corresponding HD. Moreover, we show at single-cell resolution in intact embryos that the HD Slouch (Slou) controls myoblast gene expression through unique DNA sequences that are preferentially bound by Slou. These findings demonstrate that the sequence of a HD-binding site dictates which HD family member binds to and regulates a particular enhancer. This represents a novel mechanism for how cell type-specific TFs induce the distinct genetic programs of individual embryonic cells. Overall design: Mesodermal cells were profiled from wild type, slou gain-of-function and msh gain-of-function backgrounds.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA137407
authentication:
none
authorization:
none
ID:
pmid:22296846
dateReleased:
02-08-2012
name:
Drosophila melanogaster
ncbiID:
ncbitax:7227
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • R01 HG005287/HG/NHGRI NIH HHS/United States

  • Intramural NIH HHS/United States

  • F32 GM090645/GM/NIGMS NIH HHS/United States

  • 5 T32 GM007748-31/GM/NIGMS NIH HHS/United States

  • 1 F32 GM090645-01A1/GM/NIGMS NIH HHS/United States

  • T32 GM007748/GM/NIGMS NIH HHS/United States

  • U01 GM076603/GM/NIGMS NIH HHS/United States

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