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Title: Transcriptional profiling of luteinising hormone receptor deficient mice before and after testosterone treatment      
keywords:
Transcriptome or Gene expression
ID:
PRJNA120315
description:
Luteinising hormone (LH) is a key regulator of male fertility through its effects on testosterone secretion by Leydig cells. Mice in which the LH receptor is knocked out (LuRKO) show reduced testicular size, reduced testosterone, elevated serum LH, and a spermatogenic arrest that can be rescued by administration of testosterone. This study examines the onset of spermatogenic arrest in LuRKO males using transcriptional profiling of developing mutant and control testes. We also examine the initial stages of testosterone rescue of the phenotype, in order to identify key upstream regulators of testosterone-dependent spermatogenesis. Overall design: Three experimental series were obtained: (a) a developmental time course examining LuRKO and wild-type litter-mate testes at postpartum days 3, 8, 13, 19 and 27, taking birth as day 1; (b) a “rescue” time course following the testicular response to testosterone propionate treatment (2.5mg) at 2, 4, 8, 12, 24, and 48 hours post injection; (c) a second “rescue” time course examining the dose sensitivity of the androgen response at 24hrs post injection, using varying doses of testosterone propionate (vehicle only control, 250ng, 2.5µg, 25µg and 2.5mg). For each experimental sample, two biological replicates were obtained. Each individual replicate comprised pooled testis tissue from two animals.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA120315
authentication:
none
authorization:
none
ID:
pmid:20164437
dateReleased:
12-14-2009
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject