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Title: Regulatory T cell-mediated resolution of lung injury: Identification of potential target genes via expression profiling      
keywords:
Transcriptome or Gene expression
ID:
PRJNA119073
description:
We examined early and late gene expression changes using the IT LPS model of Acute Lung Injury (ALI). In this model, injury peaks at day 4 and is almost completely resolved by day 10 in wild type (WT) C57BL/6 mice. In contrast to the pattern in WT mice, lymphocyte-deficient Rag-1 -/- mice exhibit strikingly delayed resolution despite similar initial injury. Overall design: Total RNA was isolated from mouse lung at time 0, 1, 4, and 10 days following LPS treatment of wild type and Rag-1 null mice using TRIzol reagent (Invitrogen Life Technologies), then purified using RNAeasy columns (Qiagen) with DNase I treatment according to the manufacturer’s recommendations. The quality of RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies).
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA119073
authentication:
none
authorization:
none
ID:
pmid:20028937
dateReleased:
12-31-2009
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name: