Regulatory T cell-mediated resolution of lung injury: Identification of potential target genes via expression profiling | bioCADDIE Data Discovery Index

biomedical and healthCAre Data Discovery Index Ecosystem

biomedical and healthCAre Data Discovery Index Ecosystem

Title:

Regulatory T cell-mediated resolution of lung injury: Identification of potential target genes via expression profiling

Dataset

keywords:	Transcriptome or Gene expression
ID:	PRJNA119073
description:	We examined early and late gene expression changes using the IT LPS model of Acute Lung Injury (ALI). In this model, injury peaks at day 4 and is almost completely resolved by day 10 in wild type (WT) C57BL/6 mice. In contrast to the pattern in WT mice, lymphocyte-deficient Rag-1 -/- mice exhibit strikingly delayed resolution despite similar initial injury. Overall design: Total RNA was isolated from mouse lung at time 0, 1, 4, and 10 days following LPS treatment of wild type and Rag-1 null mice using TRIzol reagent (Invitrogen Life Technologies), then purified using RNAeasy columns (Qiagen) with DNase I treatment according to the manufacturer’s recommendations. The quality of RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies).

Access

accesstypes:	download
landingpage:	http://www.ncbi.nlm.nih.gov/bioproject/PRJNA119073
authentication:	none
authorization:	none

Publication

ID:	pmid:20028937

Dataset Distribution

dateReleased:	12-31-2009

Taxonomicinformation

name:	Mus musculus
ncbiID:	ncbitax:10090

Organization

abbreviation:	NCBI
homePage:	http://www.ncbi.nlm.nih.gov
ID:	SCR:006472
name: