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Title: Functional cooperation between AAA-ATPase domain and a bromodomain.      
keywords:
Transcriptome or Gene expression
ID:
PRJNA113667
description:
A functional cooperation between an AAA type ATPase domain and a bromodomain has been evidenced. These two domains were identified and their activity tested in a yet uncharacterized factor, named Atad2, whose two-domain configuration remained highly conserved during evolution. In human and mouse, Atad2 is most abundantly expressed in testis and in the mouse. It has also been detected in post-meiotic stages and in mature spermatozoa. The AAA-ATPase domain was found necessary for protein multimerization and high affinity binding to acetylated nucleosomes by its bromodomain. The binding was found specific to acetylated histone H4 tail with a critical role for the acetylated K5 position. In vivo studies show that Atad2 behaves as a general repressor of transcription and possesses a chromatin “freezing” activity. Finally, we demonstrate that the induction of apoptosis leads to an early degradation of Atad2, which probably facilitates the action of apoptotic factors by increasing chromatin dynamics. Overall design: The experimental design includes 6 slides, corresponding to 3 experimental comparisons, in dye-swap. Each comparison corresponds is done on a specific cell line (A549, SAOS or H1299), treated with a siRNA against Atad2 or against a control siRNA.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA113667
authentication:
none
authorization:
none
ID:
pmid:20581866
dateReleased:
10-01-2009
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject