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Title: Non-coding antisense transcription detected by conventional and single-stranded cDNA microarray      
keywords:
Transcriptome or Gene expression
ID:
PRJNA99997
description:
Recent bioinformatic studies revealed that many mammalian protein-coding genes also transcribe their complementary strands. This phenomenon raises questions regarding the validity of data obtained from double-stranded cDNA microarrays since hybridization to both strands may occur. To analyze experimentally the incidence of antisense transcription in human cells and to estimate their influence on protein coding expression patterns obtained by double-stranded microarrays, we profiled transcription with sense and antisense independently by using strand-specific cDNA microarrays. Keywords: Common reference design Overall design: We modeled an experimental design typically used for tumor classification studies. We performed hybridizations using direct-labeled randomly primed total RNA extracted from eight breast-cancer cell lines and one derived from normal breast epithelium against Universal Human Reference RNA as a common reference following standard protocols.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA99997
authentication:
none
authorization:
none
ID:
pmid:17727707
dateReleased:
09-01-2007
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject