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Title: A genome-wide analysis indicates that yeast pre-mRNA splicing is predominantly post-transcriptional      
keywords:
Epigenomics
ID:
PRJNA99483
description:
Recent ChIP experiments indicate that spliceosome assembly and splicing can occur cotranscriptionally in S. cerevisiae. However, only a few genes have been examined, and all have long second exons. To extend these studies, we analyzed intron-containing genes with different second exon lengths, by ChIP as well as by whole-genome tiling arrays (ChIP-CHIP). The data indicate that U1 snRNP recruitment is independent of exon length. Recursive splicing constructs, which uncouple U1 recruitment from transcription, suggest that cotranscriptional U1 recruitment contributes to optimal splicing efficiency. In contrast, U2 snRNP recruitment as well as cotranscriptional splicing is deficient on short second exon-genes. We estimate that approximately 90% of endogenous yeast splicing is post-transcriptional, consistent with an analysis of post-transcriptional snRNP-associated pre-mRNA. Keywords: ChIP-CHIP Overall design: ChIP-CHIP was performed on tagged U1, U2, and U5 snRNPs to address spliceosome assembly on intron-containing genes in S. cerevisiae. Data expressed as log2 (immunoprecipitation/input) ratios.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA99483
authentication:
none
authorization:
none
ID:
pmid:17189193
dateReleased:
01-22-2007
name:
Saccharomyces cerevisiae
ncbiID:
ncbitax:4932
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject