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Title: The Lactococcus lactis CodY regulon: identification of a conserved cis-regulatory element      
keywords:
Transcriptome or Gene expression
ID:
PRJNA92381
description:
CodY of Lactococcus lactis MG1363 is a transcriptional regulator that represses the expression of several genes encoding proteins of the proteolytic system. DNA microarray analysis, comparing the expression profiles of L. lactis MG1363 and an isogenic strain in which codY was mutated, was used to determine the CodY regulon. In peptide-rich medium and exponentially growing cells, where CodY exerts strong repressing activity, the expression of over 30 genes was significantly increased upon removal of codY. The differentially expressed genes included those predominantly involved in amino acid transport and metabolism. In addition, several genes belonging to other functional categories were derepressed, stressing the pleiotropic role of CodY. Scrutinizing the transcriptome data with bioinformatics tools revealed the presence of a novel overrepresented motif in the upstream regions of several of the genes derepressed in L. lactis MG1363codY. Evidence is presented that this 15-bps cis-sequence, AATTTTCWGAAAATT, serves as a high-affinity binding site for CodY, as shown by electrophoretic mobility shift assays and DNaseI footprinting analyses. The presence of this CodY-box is sufficient to evoke CodY-mediated regulation in vivo. A copy of this motif is also present in the upstream region of codY itself. It is shown that CodY regulates its own synthesis and requires the CodY-box and branched-chain amino acids to interact with its promoter. Keywords: genetic modification Overall design: Each amplicon was spotted twice (technical replicates) on the DNA microarray. The RNA for wild-type and dCodY strains was isolated in 4 biological replicates which were hybridized in (partly dye-swap) to the 5 slides.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA92381
authentication:
none
authorization:
none
ID:
pmid:16040604
dateReleased:
07-22-2005
name:
Lactococcus lactis subsp. lactis Il1403
ncbiID:
ncbitax:272623
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject