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Title: Drosophila CNS mitochondrial dysfunction with Ras/MAPK inhibition microarray      
keywords:
Transcriptome or Gene expression
ID:
PRJNA355173
description:
Mitochondrial dysfunction causes biophysical, metabolic and signalling changes that alter homeostasis and reprogram cells. We used a Drosophila model in which TFAM is overexpressed in the nervous system with or without Ras/MAPK pathway inhibition, by knock-down of the ETS transcription factor pointed, to investigate the how mitochondrial dysfunction and Ras/MAPK signalling affect the transcriptome. We used microarray analysis to investigate gene expression in cases of mitochondrial dysfunction in the CNS with or without Ras/MAPK pathway inhibition by knock-down of pointed. Overall design: RNA was purified from the late third instar larval CNS from control larvae, or larvae over-expressing mitochondrial transcription factor A (TFAM) in post-mitotic neurons using the neuron specific driver nSyb-Gal4, or larvae over-expressing TFAM in combination with knock-down of pointed. Three replicates are included for the control and 4 for TFAM overexpression and TFAM overexpression combined with pointed knock-down.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA355173
authentication:
none
authorization:
none
name:
Drosophila melanogaster
ncbiID:
ncbitax:7227
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject