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Title: Microarray analysis of CurDC versus BMDC      
keywords:
Transcriptome or Gene expression
ID:
PRJNA320544
description:
We found that dendritic cells (DCs) activated by dectin-1 agonists Curdlan potently promoted naïve CD4+ T cells to differentiate into Th9 cells. To clarify the underlying mechanisms, we examine the gene expression profiles of Curdlan-activated DCs (CurDCs) and regular DCs matured by TNFalpha and IL1beta. We identified 42 upregulated genes of cytokines, chemokines and costimulatory molecules, especially the TNF/Receptor members. Subsequent studies proved that the TNF family members TNFSF15 and OX40L play a major role in mediating CurDC-induced Th9 differentiation. Overall design: Immature DCs were generated from Balb/c mice and matured with TNF-α/IL-1β (BMDCs) or Curdlan (CurDCs) for 48 hours. DCs were collected and stored in Trizol reagent (Invitrogen) at -80℃. All samples were sent to OneArray (http://www.OneArray.com.cn/, Beijing, China) for transcription profiling via genome-wide microarrays, and the subsequent data analysis was also performed by OneArray.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA320544
authentication:
none
authorization:
none
ID:
pmid:27492902
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject