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Title: Altered microRNA expression and pre-mRNA splicing events reveal new mechanisms associated with early stage Mycobacterium avium subspecies paratuberculosis infection      
keywords:
Transcriptome or Gene expression
ID:
PRJNA300273
description:
The molecular regulatory mechanisms of host responses to Mycobacterium avium subsp. paratuberculosis (MAP) infection during the early subclinical stage are still not clear. In this study, surgically isolated ileal segments in newborn calves (n = 5) were used to establish in vivo MAP infection adjacent to an uninfected control intestinal compartment. RNA-seq was used to profile the whole transcriptome (mRNAs) and the microRNAome (miRNAs) of ileal tissues collected at one-month post-infection. The most related function of the differentially expressed mRNAs between infected and uninfected tissues was “proliferation of endothelial cells”, indicating that MAP infection may lead to the over-proliferation of endothelial cells. In addition, 46.2% of detected mRNAs displayed alternative splicing events. The pre-mRNA of two genes related to macrophage maturation (monocyte to macrophage differentiation-associated) and lysosome function (adenosine deaminase) showed differential alternative splicing events, suggesting that specific changes in the pre-mRNA splicing sites may be a mechanism by which MAP escapes host immune responses. Moreover, 9 miRNAs were differentially expressed after MAP infection. The integrated analysis of microRNAome and transcriptome revealed that these miRNAs might regulate host responses to MAP infection, such as “proliferation of endothelial cells” (bta-miR-196b), “bacteria recognition” (bta-miR-146b), and “regulation of the inflammatory response” (bta-miR-146b). Overall design: Five male, Holstein calves that were 10-14 days old were inoculated with MAP using surgically isolated intestinal segments.The distal compartments were injected with 1- 3×10^8 CFU of MAP strain K10 in a final volume of 5 ml phosphate-buffered saline (PBS). The proximal compartment of the intestinal segment was injected with 5 ml PBS. The miRNA expression and mRNA expression were compared between two groups.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA300273
authentication:
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authorization:
none
ID:
pmid:27102525
name:
Bos taurus
ncbiID:
ncbitax:9913
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject