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Title: Comparison of gene expression profiles by CD3+CD4+ thymocytes derived from fetal and adult hematopoietic stem cells      
keywords:
Transcriptome or Gene expression
ID:
PRJNA142743
description:
Human fetal and adult hematopoietic stem cells (HSC) were obtained from fetal liver, fetal bone marrow (BM), and adult BM. These were injected into human fetal thymic implants in SCID-hu Thy/Liv mice (4-6 separate mice per HSC donor) and allowed to mature into single positive CD4+ (SP4) thymocytes over the course of 7-8 weeks. SP4 thymocytes from injected stem cells were subsequently sort-purified from thymic implants and gene expression was performed. Overall design: HSC from fetal (age 18-22 gestational weeks) and adult (age: 19-43 year old) HLA-A2+ donors were obtained from different tissues. After injection into human fetal thymic implants (SCID-hu Thy/Liv HLA-A2-) the cells were allowed to mature into thymocytes and sorted on the basis of HLA-A2+ expression and CD3+CD4+ (SP4) expression. 3 separate thymic implants were analyzed for each group.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA142743
authentication:
none
authorization:
none
ID:
pmid:21164017
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • OD00329/OD/NIH HHS/United States

  • R37 AI040312-15/AI/NIAID NIH HHS/United States

  • R21 AG033314/AG/NIA NIH HHS/United States

  • AI40312/AI/NIAID NIH HHS/United States

  • N01AI70002/AI/NIAID NIH HHS/United States

  • CA049605/CA/NCI NIH HHS/United States

  • P30 AI027763/AI/NIAID NIH HHS/United States

  • DP1 OD000329-05/OD/NIH HHS/United States

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