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Title: Impact of IRF1 on transcriptome changes in Huh-7 human hepatoma cells and STAT1-deficient human fibroblasts      
keywords:
Transcriptome or Gene expression
ID:
PRJNA136013
description:
IRF1, and interferon-induced transcription factor, was found to have antiviral activity against a range of viruses when overexpressed. To determine which genes are associated with expression of IRF1, gene expression analysis was carried out in two different cell line expressing IRF1. The results show that more than 100 genes, many of which overlap with type I interferon-stimulated genes, are induced more than 3-fold by IRF1. A majority of the genes were induced in both cell types, while some genes were highly induced in a cell type-specific manner. Overall design: To investigate the impact of IRF1 overexpression on the transriptome of two target cell lines, cells were transduced with a lentiviral vector expressing IRF1 or Firefly luciferase (Fluc) as a control. Total mRNA was harvested 48 h post-transduction and processed for Illumina BeadArray analysis. Skin fibroblast cell line described in Dupuis, S. et al., Impaired response to interferon-alpha/beta and lethal viral disease in human STAT1 deficiency. Nat Genet 33 (3), 388 (2003)
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA136013
authentication:
none
authorization:
none
ID:
pmid:21478870
dateReleased:
04-01-2011
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • AI064003/AI/NIAID NIH HHS/United States

  • F32 DK081193/DK/NIDDK NIH HHS/United States

  • F32 DK081193-01A1/DK/NIDDK NIH HHS/United States

  • U54 AI057158/AI/NIAID NIH HHS/United States

  • F32 DK082155-01/DK/NIDDK NIH HHS/United States

  • R01 AI064003-01/AI/NIAID NIH HHS/United States

  • Howard Hughes Medical Institute/United States

  • DK082155/DK/NIDDK NIH HHS/United States

  • U54 AI057158-01/AI/NIAID NIH HHS/United States

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