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Title: DIP-chip from Cbf1, Leu3, Pho2, Pho4, Rap1, Rox1, and Swi5      
keywords:
Epigenomics
ID:
PRJNA109407
description:
DNA Immunoprecipitation was performed using purified, naked, genomic DNA and purified recombinant DNA binding domains for S. cerevisiae transcription factors (Cbf1, Leu3, Pho2, Pho4, Rap1, Rox1, and Swi5) and then competitively hybridized against input DNA on NimbleGen 385k whole-genome, 32bp, tiling arrays to identify the consensus sequence for each transcription factor as a whole in the genome. Overall design: Each protein was used for 2 independent replicates at a protein concentration of 40nM. The second replicate is a dye-swap. During analysis, regions of the genome had to be identified as bound in both replicates as well as the average of the two replicates to be considered true binding sites.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA109407
authentication:
none
authorization:
none
ID:
pmid:19092803
dateReleased:
12-04-2008
name:
Saccharomyces cerevisiae
ncbiID:
ncbitax:4932
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • R01 CA119176/CA/NCI NIH HHS/United States

  • R01 GM072518-02/GM/NIGMS NIH HHS/United States

  • R01 GM072518-04/GM/NIGMS NIH HHS/United States

  • R01 GM054692/GM/NIGMS NIH HHS/United States

  • R01 GM054692-11/GM/NIGMS NIH HHS/United States

  • R01 GM072518-03/GM/NIGMS NIH HHS/United States

  • R01 GM058617/GM/NIGMS NIH HHS/United States

  • R01 GM058617-11/GM/NIGMS NIH HHS/United States

  • R01 GM072518/GM/NIGMS NIH HHS/United States

  • R01 GM072518-01A1/GM/NIGMS NIH HHS/United States

  • R01 CA119176-03/CA/NCI NIH HHS/United States

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