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Title: Transcription profiling of Streptomyces coelicolor transition from primary to secondary metabolism and the production of antibiotics      
dateReleased:
06-29-2005
description:
The eubacterial species Streptomyces coelicolor proceeds through a complex growth cycle in which morphological differentiation/development is associated with a transition from primary to secondary metabolism and the production of antibiotics. We used DNA microarrays and mutational analysis to investigate the expression of individual genes and multigene antibiotic biosynthetic pathways during these events. We identified expression patterns in biosynthetic, regulatory, and ribosomal protein genes that were associated highly specifically with particular stages of development. A knowledge-based algorithm that correlates temporal changes in expression with chromosomal position identified groups of contiguous genes expressed at discrete stages of morphological development, inferred the boundaries of known antibiotic synthesis gene loci, and revealed novel physical clusters of coordinately regulated genes. Microarray analysis of RNA from cells mutated in genes regulating synthesis of the antibiotics actinorhodin (Act) and undecylprodigiosin (Red) identified proximate and distant sites that contain putative ABC transporter and two-component system genes expressed coordinately with genes of specific biosynthetic pathways and indicated the existence of two functionally and physically discrete regulons in the Red pathway.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-SMDB-837
refinement:
raw
dateSubmitted:
06-30-2005
keywords:
functional genomics
dateModified:
06-04-2014
availability:
available
types:
gene expression
name:
Streptomyces coelicolor
name:
unknown experiment type
ID:
A-SMDB-396
name:
SMD Streptomyces coelicolor Array STREP-2
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-SMDB-837/E-SMDB-837.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-SMDB-837/E-SMDB-837.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress