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Title: Transcription profiling of mouse embyonic fibroblasts from wild type and Elavl1knock-out embryos      
dateReleased:
03-15-2009
description:
Mouse embryonic fibroblasts (MEF) were isolated via enzymatic dissociation of 3 E14.5 embrya bearing epiblast specific inactivation of the murine Elavl1 locus as 3 littermate controls. Individual Fibroblast cultures were maintained in complete DMEM medium containing 10%FBS at 370C with 5%CO2. Cultures were passaged for 3 times and harvested for RNA isolation when they reached 60-70% conflunecy.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-1785
refinement:
raw
keywords:
functional genomics
dateModified:
05-02-2014
creators:
Vaggelis Harokopos
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-AFFY-130
name:
Affymetrix GeneChip Mouse Gene 1.0 ST Array [MoGene-1_0-st-v1]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-1785/E-MEXP-1785.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-1785/E-MEXP-1785.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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