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Title: Transcription profiling of human HEL cells treated with either 5 uM of JI1 or 10 uM of Erlotinib reveal growth of leukemic Jak2-V617F positive cells is mutually suppressed by Jak inhibitors and Erlotinib through distinct mechanisms      
dateReleased:
08-10-2009
description:
Gene expression profiling experiments were performed using the Agilent 44K whole human genome microarrays (Agilent Biotechnologies, Diegem, Belgium) according to the two-color gene expression analysis (Quick Amp labeling) protocol (version 5.7) from the manufacturer. Hybridizations were performed by comparing 300 ng of total RNA extracted from HEL cells treated with either 5 ?M of JI1 or 10 ?M of Erlotinib with that obtained from untreated cells. For each treatment, three biological replicates were analyzed in duplicate including a dye swap. Only high quality RNAs with a ribosomal RNA ratio greater than 1.9 and no evidence of degradation, as evaluated using the Agilent Bioanalyzer 2100 RNA 6000 nano assay, were used in this study. Microarray images were quantified using the GenePix Pro 6.1 software (Molecular Devices, Sunnyvale, CA, USA) as described in supplemental information.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MEXP-2244
refinement:
raw
dateSubmitted:
07-08-2009
keywords:
functional genomics
dateModified:
05-02-2014
creators:
Arnaud Muller
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-MEXP-1125
name:
Agilent Whole Human Genome Microarray 4x44K 014850 G4112F (266 cols x 170 rows)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-2244/E-MEXP-2244.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MEXP-2244/E-MEXP-2244.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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