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Title: Transcription profiling of E. coli HMS174(DE3)(pET30aNproGFPmut3.1) to analyze cellular response to full induction with IPTG      
dateReleased:
03-26-2010
description:
This transcription profiling time course experiment was conducted in order to analyze the cellular response of the T7 RNA polymerase based E. coli expression system HMS174(DE3)(pET30aNproGFP) to a single pulse induction with isopropyl-beta-D-galactoside (IPTG). The used cell material was produced under well controlled and defined conditions in an exponential carbon limited fed-batch cultivation at a growth rate of 0.1 per hour. The applied amount of 20µmol IPTG per gram cell dry weight assured a fully induced system and high recombinant gene expression rates. Non induced cells sampled at the time point of induction were used as reference and compared to cells subsequently sampled during the production period (sampling frequency 1 hour)
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MARS-16
refinement:
raw
keywords:
functional genomics
dateModified:
10-12-2011
availability:
available
types:
gene expression
name:
Escherichia coli
name:
time series
ID:
A-MARS-10
name:
MARS acbt_ecoli_k12_2005_rev20090226
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MARS-16/E-MARS-16.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MARS-16/E-MARS-16.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress