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Title: Exon array analysis of resting and activated primary murine CD4+ T-cells      
dateReleased:
05-26-2010
description:
The stability, localization and translation of mammalian mRNAs are largely determined by sequences in the 3' untranslated region (UTR). Here, we describe a conserved program of increased upstream polyadenylation site usage following activation of primary murine CD4+ T lymphocytes. This program, resulting in shorter 3' UTRs, is a characteristic of immune cell activation and strongly correlates with proliferation across diverse cell types and tissues. Enforced expression of full-length 3' UTRs confers differences in protein expression that can in some cases be eliminated by deletion of predicted microRNA target sites in the variably included region. Together, our data indicate that polyadenylation site usage is coordinately regulated such that states of increased proliferation are associated with widespread reduction of 3' UTR-based regulation. Keywords: activation, exon array, time course Two or three biological replicates per time point
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-10666
refinement:
raw
alternateIdentifiers:
10666
dateSubmitted:
02-28-2008
keywords:
functional genomics
dateModified:
03-27-2012
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-GEOD-6538
name:
Alternative Mouse Exon 1.0 ST Array [CDF: MmEx10stv1_Mm_REFSEQ_8]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-10666/E-GEOD-10666.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-10666/E-GEOD-10666.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE10666
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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