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Title: Expression data from Burkholderia multivorans cystic fibrosis clinical isolates      
dateReleased:
08-21-2011
description:
Burkholderia cepacia complex (Bcc) comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation. Here we studied two Burkholderia multivorans clonal isolates (D2095 and D2214) displaying different morphotypes from a chronically infected CF patient in order to evaluate traits development during lung infection. For expression profiling, overnight cultures of the mucoid D2095 and the nonmucoid D2214 isolates grown in SM medium were diluted to an initial OD640 nm of 0.1 into the same medium. Triplicate samples were cultured at 37ºC with 250 r.p.m. agitation for 17 h and RNA extracted from the two bacterial isolates.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-28306
refinement:
raw
alternateIdentifiers:
28306
keywords:
functional genomics
dateModified:
09-06-2011
availability:
available
types:
gene expression
name:
Burkholderia multivorans
ID:
A-GEOD-13356
name:
Affymetrix IST_IBB Burkholderia 15K v1.0 (GeneChip BCC1sa520656F)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-28306/E-GEOD-28306.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-28306/E-GEOD-28306.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE28306
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress