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Title: Hybridization of Burkholderia multivorans D2095 and D2214 genomic DNA      
dateReleased:
08-21-2011
description:
Burkholderia cepacia complex (Bcc) comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation. Here we studied two Burkholderia multivorans clonal isolates (D2095 and D2214) displaying different morphotypes from a chronically infected CF patient in order to evaluate traits development during lung infection. Since the custom array described in platform GPL13356 was based on Burkholderia multivorans ATCC 17616 genome, here we performed a DNA-DNA hybridization to determine which probes of the array hybridize with our test genomes Total DNA was extracted from overnight cultures of the mucoid D2095 and the nonmucoid D2214 isolates grown in LB medium.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-30402
refinement:
raw
alternateIdentifiers:
30402
keywords:
functional genomics
dateModified:
09-06-2011
availability:
available
types:
gene expression
name:
Burkholderia multivorans
ID:
A-GEOD-13356
name:
Affymetrix IST_IBB Burkholderia 15K v1.0 (GeneChip BCC1sa520656F)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-30402/E-GEOD-30402.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-30402/E-GEOD-30402.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30402
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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