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Title: Transcription profiling of E. coli BL21(DE3) to analyze the cellular response to high level expression of recombinant human super-oxide-dismutase (SOD)      
dateReleased:
09-30-2011
description:
This transcription profiling time course experiment was conducted in order to analyze the cellular response of E. coli BL21(DE3) to high level expression of recombinant human super-oxide-dismutase (SOD) on the transcriptional level. Three biological replicates were generated by using a carbon limited exponential fed-batch cultivation similar to industrial setups for large scale production. For induction of the system a single pulse of isopropyl-beta-D-galactoside (IPTG) yielding in a fully induced system is applied one doubling past feed start. In order to achieve a proper timeresolution of cellular responses sampling starts with a high frequency for the first hours past induction and decreases in the course of the experiment.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MARS-19
refinement:
raw
keywords:
functional genomics
dateModified:
05-31-2013
availability:
available
types:
gene expression
name:
Escherichia coli
ID:
A-MARS-11
name:
MARS acbt_ecoli_bl21_2007
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MARS-19/E-MARS-19.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MARS-19/E-MARS-19.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress